Protecting biodiversity and economic returns in resource-rich tropical forests.

In pursuit of socioeconomic development, many countries are expanding oil and mineral extraction into tropical forests. These activities seed access to remote, biologically rich areas, thereby endangering global biodiversity. Here we demonstrate that conservation solutions that effectively balance the protection of biodiversity and economic revenues are possible in biologically valuable regions.

Using spatial data on oil profits and predicted species and ecosystem extents, we optimise the protection of 741 terrestrial species and 20 ecosystems of the Ecuadorian Amazon, across a range of opportunity costs (i.e. sacrifices of extractive profit). For such an optimisation, giving up 5% of a year’s oil profits (US$ 221 million) allows for a protected area network that retains of an average of 65% of the extent of each species/ecosystem.

This performance far exceeds that of the network produced by simple land area optimisation which requires a sacrifice of approximately 40% of annual oil profits (US$ 1.7 billion), and uses only marginally less land, to achieve equivalent levels of ecological protection.

Applying spatial statistics to remotely sensed, historic deforestation data, we further focus the optimisation to areas most threatened by imminent forest loss. We identify Emergency Conservation Targets: areas that are essential to a cost-effective conservation reserve network and at imminent risk of destruction, thus requiring urgent and effective protection.

Governments should employ the methods presented here when considering extractive led development options, to responsibly manage the associated ecological-economic trade-offs and protect natural capital. Article Impact Statement: Governments controlling resource extraction from tropical forests can arrange production and conservation to retain biodiversity and profits. This article is protected by copyright. All rights reserved.

Three ways to deliver a net positive impact with biodiversity offsets.

Biodiversity offsetting is the practice of using conservation actions such as habitat restoration, management or protection to compensate for ecological losses caused by development activity, including construction projects. The typical goal of offsetting is no net loss (NNL), which means that all ecological losses are compensated for by commensurate offset gains.

The focus of this work is a conceptual and methodological exploration of net positive impact (NPI), an ambitious goal that implies commitment to beyond NNL, and which has recently received increasing attention from both big business and environmental NGOs. We build upon three ways to deliver NPI:

(i) use of an additional NPI multiplier, (ii) using permanent offsets to deliver additional gains after NNL has first been reached during a shorter offset evaluation time interval, and a (iii) novel modification of the mitigation hierarchy so that gains from its traditional third step, onsite rehabilitation, can no longer be counted towards reduced offset requirements.

DMEM, High Glucose, No L-glutamine, No sodium pyruvate

CM007-300 GenDepot 6x500ml 165 EUR

DMEM, High Glucose, No L-glutamine, No sodium pyruvate

CM007-310 GenDepot 10x500ml 205 EUR

DMEM, High Glucose, No L-glutamine, No sodium pyruvate

CM007-320 GenDepot 20x500ml 317 EUR

DMEM, High Glucose, No L-glutamine, No sodium pyruvate

CM007-350 GenDepot 50x500ml 585 EUR

DMEM, High Glucose , No L-glutamine, No sodium pyruvate

CM009-050 GenDepot 500ml 74 EUR

DMEM, High Glucose , No L-glutamine, No sodium pyruvate

CM009-300 GenDepot 6x500ml 165 EUR

DMEM, High Glucose , No L-glutamine, No sodium pyruvate

CM009-310 GenDepot 10x500ml 205 EUR

DMEM, High Glucose , No L-glutamine, No sodium pyruvate

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DMEM, High Glucose , No L-glutamine, No sodium pyruvate

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DMEM, High Glucose, No L-glutamine, No sodium pyruvate

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DMEM, High Glucose, No L-glutamine, No sodium pyruvate

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DMEM, High Glucose, No L-glutamine, No sodium pyruvate

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CMV-Luciferase (No marker)

LVP911a GenTarget 1x107 IFU/ml x 200ul 349 EUR
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EF1a-Luciferase (No marker)

LVP911b GenTarget 1x107 IFU/ml x 200ul 349 EUR
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Sheep NO ELISA Kit

ESN0048 Abclonal 96Tests 521 EUR

Anserini NO ELISA Kit

EAN0048 Abclonal 96Tests 521 EUR

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ERN0048 Abclonal 96Tests 521 EUR

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ERTN0048 Abclonal 96Tests 521 EUR

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EMKN0048 Abclonal 96Tests 521 EUR

Mouse NO ELISA Kit

EMN0048 Abclonal 96Tests 521 EUR

Porcine NO ELISA Kit

EPN0048 Abclonal 96Tests 521 EUR

Human NO ELISA Kit

EHN0048 Abclonal 96Tests 521 EUR

Chicken NO ELISA Kit

ECKN0048 Abclonal 96Tests 521 EUR

Canine NO ELISA Kit

ECN0048 Abclonal 96Tests 521 EUR

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EBN0048 Abclonal 96Tests 521 EUR

Goat NO ELISA Kit

EGTN0048 Abclonal 96Tests 521 EUR

Anti-NO-synthase antibody

STJ16101278 St John's Laboratory 50 µl 964 EUR

Anti-NO-synthase antibody

STJ16101290 St John's Laboratory 50 µl 949 EUR

Anti-NO-synthase antibody

STJ16101412 St John's Laboratory 50 µl 964 EUR

Anti-NO-synthase antibody

STJ16101424 St John's Laboratory 50 µl 949 EUR

Ceraplex , No Phenol red

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SEQ ID NO:549

5-01922 CHI Scientific 4 x 5mg Ask for price

NO ELISA KIT|Human

EF007402 Lifescience Market 96 Tests 689 EUR

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CM020-050 GenDepot 500ml 85 EUR

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DMEM/Ham's F-12 (1:1 Mixture), No HEPES, No Phenol Red

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DMEM/Ham's F-12 (1:1 Mixture), No HEPES, No Phenol Red

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DMEM/Ham's F-12 (1:1 Mixture), No HEPES, No Phenol Red

CM020-350 GenDepot 50x500ml 585 EUR

2.0ML MICROTUBES, NO CAP. CLEAR

MCT-200-NC CORNING 500/pk 111 EUR
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cellulose acetate unit (no accessories!)

EHCA1100-SYS Consort ea 606 EUR

Guinea Pig NO ELISA Kit

EGN0048 Abclonal 96Tests 521 EUR

SensiMix SYBR No-ROX Kit

QT650-05 Bioline 500 rxn Ask for price

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Agar, Bacto , Cat No Changed

A2007-510 GenDepot 10Kg 2102 EUR

SensiFAST Probe No-ROX Kit

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SensiFAST SYBR No-ROX Kit

BIO-98005 Bioline 500 rxns Ask for price

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BIO-98020 Bioline 2000 rxns Ask for price

SEQ ID NO:549 Peptide

20-abx266211 Abbexa
  • 439.00 EUR
  • 718.00 EUR
  • 328.00 EUR
  • 10 mg
  • 25 mg
  • 5 mg

TARGATT? 3 (no promoter - MCS)

AST-3043 Applied StemCell 20 uL 275 EUR
Description: 12 month

BLOOD AGAR BASE NO.2

B02-109-10kg Alphabiosciences 10 kg 1266 EUR

BLOOD AGAR BASE NO.2

B02-109-2Kg Alphabiosciences 2 Kg 314 EUR

BLOOD AGAR BASE NO.2

B02-109-500g Alphabiosciences 500 g 122 EUR

Rabbit Anti-Human Ferritin (H-chain) IgG # 3, aff pure (no azide/no bsa)

FERH13-AW Alpha Diagnostics 100 ug 445 EUR

96 well filter plate (no filter)

SD5006-EMPTY Bio Basic 12UNIT 87.41 EUR

Rabbit nitric oxide,NO ELISA Kit

GA-E0152RB-48T GenAsia Biotech 48T 326 EUR

Rabbit nitric oxide,NO ELISA Kit

GA-E0152RB-96T GenAsia Biotech 96T 524 EUR

Mouse nitric oxide(NO)ELISA Kit      

GA-E0452MS-48T GenAsia Biotech 48T 336 EUR

Mouse nitric oxide(NO)ELISA Kit      

GA-E0452MS-96T GenAsia Biotech 96T 534 EUR

Rat nitric oxide(NO)ELISA Kit

GA-E0711RT-48T GenAsia Biotech 48T 373 EUR

Rat nitric oxide(NO)ELISA Kit

GA-E0711RT-96T GenAsia Biotech 96T 609 EUR

Human nitric oxide(NO)ELISA Kit

GA-E1527HM-48T GenAsia Biotech 48T 289 EUR

Human nitric oxide(NO)ELISA Kit

GA-E1527HM-96T GenAsia Biotech 96T 466 EUR

HotTaq Probe qPCR Mix (no ROX)

BT11002 Bioatlas 250rxn 109 EUR
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

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BT11102 Bioatlas 250rxn 123 EUR
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BT11202 Bioatlas 250rxn 123 EUR
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Human nitric oxide,NO ELISA Kit

201-12-1511 SunredBio 96 tests 440 EUR
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Genomic DNA Kit (No RNase A)

20-abx098862 Abbexa
  • 398.00 EUR
  • 230.00 EUR
  • 200 rxns
  • 50 rxns

Rabbit nitric oxide,NO ELISA Kit

QY-E30147 Qayee Biotechnology 96T 374 EUR

Chicken Nitric Oxide, NO ELISA Kit

QY-E80065 Qayee Biotechnology 96T 426 EUR

Rat nitric oxide(NO)ELISA Kit

QY-E10941 Qayee Biotechnology 96T 361 EUR

Rat nitric oxide (NO) ELISA Kit

QY-E11842 Qayee Biotechnology 96T 374 EUR

Mouse nitric oxide(NO)ELISA Kit

QY-E21092 Qayee Biotechnology 96T 361 EUR

Human nitric oxide(NO)ELISA Kit

QY-E00533 Qayee Biotechnology 96T 361 EUR

TaqProbe 5X qPCR MasterMix-No Dye

MasterMix-5PS ABM 4 x 1.0 ml for 1000 reactions (20 ul) 213 EUR

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MasterMix-mS ABM 4 x 1.25 ml for 500 reactions 151 EUR

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MasterMix-PS ABM 4 x 1.25 ml for 500 reactions (20 ul) 140 EUR

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BrightGreen 2X qPCR MasterMix-No Dye

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BIO-11060 Cusabio 10 x 10ml Ask for price

hTERT (EF1a) lentivirus (No any antibiotic selection)

LVP1131 GenTarget 1x107 IFU/ml x 200ul 349 EUR
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LVP911a-PBS GenTarget 5x107 IFU/ml x 200ul 710 EUR
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LVP911b-PBS GenTarget 5x107 IFU/ml x 200ul 710 EUR
Description: Pre-made lentivirus express firefly luciferase under EF1a promoter (do not containing any antibiotic selection marker), concentrated and provided in PBS solution

Grace's With L-glutamine. No sodium bicarbonate.

GIP05-10LT Caisson Labs 10 L 101 EUR
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Grace's With L-glutamine. No sodium bicarbonate.

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Description: With L-glutamine. No sodium bicarbonate.

Grace's With L-glutamine. No sodium bicarbonate.

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KA009 Sceti 596 EUR
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Anti Nitrotyrosine Monoclonal Antibody (Clone No.2H1)

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Anti PEDF Monoclonal Antibody (Clone No.14K)

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SensiFAST Lyo-Ready No-ROX Mix, 2x

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DMEM, High Glucose , Powder , No Sodiim bicarbonate

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Bacteria Genomic DNA Kit (No RNase A)

abx098240-50rxns Abbexa 50 rxns 230 EUR

Plant Genomic DNA Kit(No RNase A)

20-abx098863 Abbexa
  • 384.00 EUR
  • 230.00 EUR
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (No RNase A)

20-abx098864 Abbexa
  • 384.00 EUR
  • 230.00 EUR
  • 200 rxns
  • 50 rxns

1000bp/1kb DNA ladder (no loading dye)

305-005 GeneOn 50µg/250µl 50 EUR

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Recombinant purified human Ubiquitin protein (No-tag)

UBQ16-R Alpha Diagnostics 1 mg 202 EUR

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Porcine a nitric oxide (NO) ELISA Kit

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Guinea pig nitric oxide(NO)ELISA Kit

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One-Step BrightGreen qRT-PCR-No Dye

G471-S ABM 100 rxn (20 ul/rxn) 158 EUR

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0.2ML THIN WALL PCR TUBES, NO CAP. CLEAR

PCR-02-NC CORNING 1000/pk 318 EUR
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OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric)

STA-800 Cell Biolabs 96 assays 490 EUR
Description: The OxiSelect Intracellular Nitric Oxide Assay Kit is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells.  In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate.  When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog.  The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM).  The kit?s provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters.  The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection.  Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards, and unknown samples.

OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric)

STA-800-5 Cell Biolabs 5 x 96 assays 1923 EUR
Description: The OxiSelect Intracellular Nitric Oxide Assay Kit is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells.  In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate.  When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog.  The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM).  The kit?s provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters.  The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection.  Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards, and unknown samples.

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Anti AGE-4 Monoclonal Antibody (Clone No. 14B5)

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Anti Human RAGE Monoclonal Antibody (Clone No. 1C5)

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Anti Human GPR120 Monoclonal Antibody (Clone No. 2B6)

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Anti Human KOR Monoclonal Antibody (Clone No. 5E3)

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Anti Human DOR Monoclonal Antibody (Clone No. 1E7)

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Anti Carp Vitellogenin Monoclonal Antibody (Clone No. 1G2)

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Anti Mummichog Vitellogenin Monoclonal Antibody (Clone No. 3E11)

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Anti Medaka Vitellogenin Monoclonal Antibody (Clone No.3C1)

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Anti CML Monoclonal Antibody (Clone No. CMS-10)

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Anti CML Monoclonal Antibody (Clone No. NF-1G)

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Anti CEL Monoclonal Antibody (Clone No. KNH-30)

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Anti Rat MFH Monoclonal Antibody (Clone No. A3)

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1-Drop PCR Mix (squeeze bottle, no pipet)

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2845-18 CORNING 100/pk 112 EUR
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2845-22 CORNING 100/pk 112 EUR
Description: Cover Glass; Cover Glass, CGW

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2855-18 CORNING 50/pk 83 EUR
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COVER GLASS, NO. 2, SQUARE 22 X 22MM

2855-22 CORNING 50/pk 109 EUR
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COVER GLASS, NO. 1, RECTANGLE 22 X 30MM

2975-223 CORNING 100/pk 114 EUR
Description: Cover Glass; Cover Glass, CGW

COVER GLASS, NO. 1, RECTANGLE 22 X 40MM

2975-224 CORNING 100/pk 142 EUR
Description: Cover Glass; Cover Glass, CGW

COVER GLASS, NO. 1, RECTANGLE 22 X 50MM

2975-225 CORNING 100/pk 167 EUR
Description: Cover Glass; Cover Glass, CGW

COVER GLASS, NO. 1, RECTANGLE 24 X 30MM

2975-243 CORNING 100/pk 121 EUR
Description: Cover Glass; Cover Glass, CGW

COVER GLASS, NO. 1, RECTANGLE 24 X 40MM

2975-244 CORNING 100/pk 152 EUR
Description: Cover Glass; Cover Glass, CGW

COVER GLASS, NO. 1, RECTANGLE 24 X 50MM

2975-245 CORNING 100/pk 182 EUR
Description: Cover Glass; Cover Glass, CGW

COVER GLASS, NO. 1, RECTANGLE 24 X 60MM

2975-246 CORNING 100/pk 260 EUR
Description: Cover Glass; Cover Glass, CGW

CENTRIFUGE TUBE,50ML,NO CAP,S,25/500

431472 CORNING 25/pk 119 EUR
Description: Centrifuge Tubes; Corning Centrifuge Tubes

Marine Animal Genomic DNA Kit (No RNase A)

abx298012-50rxns Abbexa 50 rxns 230 EUR

Human Topoisomerase I ICE Assay Kit (No Antibody)

TG1020-0 TopoGen 24 Tests 425 EUR

Recombinant (E.Coli) HIV-1 p24 >95% (no tag)

RP-536 Alpha Diagnostics 10 ug 286 EUR

ExCellenCT One-Step BrightGreen qRT-PCR-No Dye

G917-S ABM 25 Preps 100 x 20 ul reactions 177 EUR

ExCellenCT One-Step TaqProbe qRT-PCR-No Dye

G918-PS ABM 25 Preps 100 x 20 ul reactions 177 EUR

EZH2-no GFP sgRNA CRISPR Lentivector set (Mouse)

K3005201 ABM 3 x 1.0 ug 339 EUR

EED-no GFP sgRNA CRISPR Lentivector set (Mouse)

K3005301 ABM 3 x 1.0 ug 339 EUR

NO ELISA Kit| Rat Nitric Oxide ELISA Kit

EF018222 Lifescience Market 96 Tests 689 EUR

Screen Quest™ FRET No Wash cAMP Assay Kit

36379 AAT Bioquest 1 plate 480 EUR

Screen Quest™ FRET No Wash cAMP Assay Kit

36380 AAT Bioquest 10 plates 789 EUR

Screen Quest™ FRET No Wash cAMP Assay Kit

36381 AAT Bioquest 50 plates 3095 EUR

Screen Quest™ No Wash Potassium Channel Assay Kit

36550 AAT Bioquest 1 Plate 219 EUR

Screen Quest™ No Wash Potassium Channel Assay Kit

36551 AAT Bioquest 10 Plates 441 EUR

Screen Quest™ No Wash Potassium Channel Assay Kit

36552 AAT Bioquest 100 Plates 3051 EUR

Green-2-Go qPCR Mastermix-no Dye (500X20ul Rxn)

QPCR006-NODYE Bio Basic 4X1.25ml 271.85 EUR

hTERT (EF1a) lentivirus in PBS (No any antibiotic selection)

LVP1131-PBS GenTarget 1x108 IFU/ml x 200ul 710 EUR
Description: Concentrated lentivirus in PBS, expressing human TERT gene under EF1a promoter. It does not contain any antibiotic selection.

Ham's F-12 With L-glutamine. No phenol red.

HFL05-500ML Caisson Labs 500 ml 75 EUR
Description: With L-glutamine. No phenol red.

Ham's F-12 With L-glutamine. No phenol red.

HFL05-6X500ML Caisson Labs 6 x 500 ml 123 EUR
Description: With L-glutamine. No phenol red.

IPL-41 With L-glutamine. No tryptose phosphate broth.

IPL01-500ML Caisson Labs 500 ml 109 EUR
Description: With L-glutamine. No tryptose phosphate broth.

IPL-42 With L-glutamine. No tryptose phosphate broth.

IPL01-6X500ML Caisson Labs 6 x 500 ml 308 EUR
Description: With L-glutamine. No tryptose phosphate broth.

DMEM/F-12 With L-glutamine. No phenol red.

DFL16-500ML Caisson Labs 500 ml 75 EUR
Description: With L-glutamine. No phenol red.

DMEM/F-12 With L-glutamine. No phenol red.

DFL16-6X500ML Caisson Labs 6 x 500 ml 124 EUR
Description: With L-glutamine. No phenol red.

DMEM/F-12 With L-glutamine. No sodium bicarbonate.

DFP19-10LT Caisson Labs 10 L 74 EUR
Description: With L-glutamine. No sodium bicarbonate.

DMEM/F-12 With L-glutamine. No sodium bicarbonate.

DFP19-10X1LT Caisson Labs 10 x 1 L 83 EUR
Description: With L-glutamine. No sodium bicarbonate.

DMEM/F-12 With L-glutamine. No sodium bicarbonate.

DFP19-50LT Caisson Labs 50 L 104 EUR
Description: With L-glutamine. No sodium bicarbonate.

amfiSure qGreen Q-PCR Master Mix(2X), No Rox

Q5600-001 GenDepot 1ml 146 EUR

amfiSure qGreen Q-PCR Master Mix(2X), No Rox

Q5600-002 GenDepot 2x1ml 207 EUR

The outcome from these three factors is that for the same ecological damage, larger offsets will be required than before, and earlier, thereby necessarily improving the success expected from offsets. As a corollary, we show how offsets really are NNL only at one ephemeral point in time, before which they are net negative and after which they turn into either net positive impact or net negative impact, depending on whether permanent offsets are combined with partially temporary losses or if temporary offset gains are combined with partially permanent losses. While we provide operational guidance on how to achieve NPI, it should be understood that achieving it is fully conditional on prior achievement of NNL, and offsets have indeed been frequently observed to fail due to inadequate policy requirements, incompetent planning or incomplete implementation.

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